Peptide Protein Research PPR Ltd
A benefit of liquid-phase synthesis, though, is that because the product is purified after each step, side reactions are easily detected. Additionally, convergent synthesis can be performed, in which separate peptides are synthesized and then coupled together to create larger peptides. Once peptide synthesis is completed, all remaining protecting groups are removed from the nascent peptides.
- If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.
- Mass spectroscopy analysis is performed to in order to confirm the identity of the final product and the mass analysis is supplied with the peptide.
- Additionally, this approach requires another chemical group to protect the C-terminus of the first amino acid.
- Solid-Phase Peptide Synthesis (SPPS), developed in the late 1950s by Robert Bruce Merrifield, uses well-established Fmoc/tBu chemistry to anchor successive amino acids onto a growing chain immobilised on a polymeric support.
- As an additional service, we can conjugate your chosen peptide to a carrier protein such as Keyhole Limpet Hemocyanin (KLH) or Bovine Serum Albumin (BSA).
Fluorescent labelled peptides
They also have state of the artPeptide Library Synthesis for hundreds of peptides. Amino acid analysis is usually performed when the concentration cannot be determined by UV absorbance due to the lack of aromatic tryptophan and tyrosine residues in the proteins or peptides. Although amino acid analysis requires specialist equipment, it is a robust and highly sensitive method that quantifies the concentration each individual amino acid in a sample.
Introduction to peptide synthesis
It is a two-step cyclical process where the Fmoc protecting group from the amino acid immobilised onto the resin is first cleaved to expose the amine group. The resulting exposed amine is then reacted with the carboxylic anabolic steroids acid group of a Fmoc protected amino acid to form an amide bond (peptide bond) using coupling reagents or activators. The deprotection and coupling steps are then repeated until the chain is assembled.
N-terminal, C-terminal and side chain modifications
‘Swell factor’ is important in SPPS because resins which swell more have a higher surface area, allowing reagents into the core of the resin more easily, speeding up the reaction and increasing the yield. Our custom peptide synthesis service offers high-quality, reliable and cost-effective products to support your research. We have unrivalled expertise in the synthesis of complex, constrained and other special peptides and our scientists are happy to assist in selecting the most appropriate peptide specification for your application. For peptide purification, RPC separates the target peptides from impurities from the synthesis steps, such as isomers, deletion sequences, peptide products from side reactions with free coupling and protecting groups or peptides that have undergone side-chain reactions. Amino acid side chains represent a broad range of functional groups and are therefore a site of considerable side chain reactivity during peptide synthesis.